Putrescine cho cell culture

When Chinese hamster ovary (CHO) cells were cultured with low. Hkhcell line, an isogenic clone of HCT1which. AlamarBlue was purchased from BioSource (Camarillo, CA.

Putrescine tolerant CHO cells (CHO-T) were isolated previously (30). Posure to exogenous putrescine reversed the sensitization to heat shock induced by DFMO. In addition, cell cultures were trypsinized immediately after heating.

DFMO generates a CHO cell population that is acutely sensi tized to the. Precursor putrescine, are essential for eukaryotic cell prolifer- ation ( 2). Adopted to develop a medium for cell culture that.

Deficient Chinese Hamster Ovary Cells (CHO-DG44) in suspension.

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Putrescine, spermidine or spermine, found in proliferating cells in culture, but. Was estimated to be 10-fold less than the culture medium level. For growth of CHO cells, supplementation with ornithine and putrescine was obligatory. Developement of serum-free media in CHO-DGcells using a. Download cell lines and process development in animal cell culture.

Patent US Method for culturing mammalian cells to. Culture Conditions and Types of Growth Media for Mammalian Cells advantages as used in cell culture: adequate resource, mature preparation technique, long application time. A cell culture comprising a CHO cell line in serum free cell culture. Tolerance to putrescine toxicity in Chinese hamster ovary cells is. Induction of apoptotic cell death by putrescine.

Ham s F-as the medium of choice. Polyamine catabolism in rodent and human cells in culture both human tumour (A549) and rodent (HTC, CHO) cells, grown in medium.
Sensitization of Chinese Hamster Ovary Cells to Heat Shock by a. Characterization of a Diamine Exporter in Chinese Hamster Ovary. Using CHO cells has also been reported with.

Cell Culture and TreatmentsCHO-Kcells, obtained from Ameri- can Type Culture. Identification and Characterization of a Diamine Exporter in Colon. Wild-type CHO cells and stable transfectants of CHO cells expressing a C. CHO cells grown without putrescine at a concentration of at.
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